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Sequencing by Ligation Variation with Endonuclease V, Deoxyinosine, and SAWTooth The Sequencing Analysis Workbench Tool

Sequencing by Ligation

Sequencing by Ligation

Cyclic SBL involves using Endonuclease V to recognize an incorporated deoxyinosine site and clip the DNA. This results in a fragment of the query primer attached to the anchor primer, allowing subsequent ligations to sequence further out.

Credits: Antoine Ho – University of New Mexico