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Microfluidic Device for High Throughput RNA Splice Variation Studies
Fig. 2. Fluorescent probing of exons on immobilized cDNA
The green fluorescence (FITC) probe is a control to verify the ligation of the cDNA to the bead via a primer, while the red (Cy5) fluorescence indicates the presence of exon 9 in the c-Myb gene. The bottom image is the overlay of these colors.
Credits: Nick Carroll, University of New Mexico
