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Investigating the Characteristics of Confined Cellular Systems on the Effectiveness of Ultra-Stable Vaccines for Mycobacterium tuberculosis.

Growth of films aged for 16 months in dry storage.

Growth of films aged for 16 months in dry storage.

Growth of M. bovis BCG aged in dark at 37 C for 16 months and then placed film-down upon 7H11 agar (with 0.5% Tween, 0.2% glycerol, and albumin-dextrose-catalase supplement) and incubated for 3 weeks. Florid mycobacterial growth is visible for cells maintained within nanostructured matrices, especially the thick cast films. Non-nanostructure encased mycobacteria were sterile.

Credits: Dr. Eric Carnes, UNM