This proposal describes an inter-institutional
IGERT program in soft materials to be offered jointly at City College of
CUNY and Columbia University, which are in close proximity. De Gennes defines soft materials as organic media that organize on supramolecular length scales via weak associative interactions. Examples include thin polymeric films for sensors, organic blends for electronic displays, and natural and artificial tissue, spanning the range from complex fluids to soft solids. They are intrinsically multi-scale, molecular to macroscopic. A stunning variety of structures and morphologies can emerge, enabling a wide range of new technologies, and all scales impact processing and use. The Ph.D. program will be built around joint seminars, an industrial internship, collaborative thesis research, and a core of joint course offerings, including two new laboratory courses. The research will be in three complementary areas: effects of confinement on polymer statics and dynamics, chemical and physical patterning, and multi-scale phenomena in bio-related materials. The proposed core curriculum will be taken in addition to the core disciplinary requirements in the student’s major department. Our proposal is consistent with existing Ph.D. requirements for all five participating departments, and does not add in any way to the degree requirements or faculty teaching loads; the soft materials courses will satisfy existing elective requirements, but will enable the students to focus these electives within a coordinated program. The proposed collaborative program is unique in two respects: First, it leverages the intellectual and material resources of two neighboring, urban, public/private institutions with complementary faculty and diverse student bodies. Second, it creates a unique, synergistic environment for research and graduate education in a niche area that is at the heart of numerous technologies essential to industries based in the greater New York regional area.
IGERT is an
NSF-wide program intended to meet the challenges of educating U.S. Ph.D. scientists and engineers with the multidisciplinary backgrounds and the technical, professional, and personal skills needed for the career demands of the future. The program is intended to catalyze a cultural change in graduate education by establishing innovative new models for graduate education and training in a fertile environment for collaborative research that transcends traditional disciplinary boundaries. In the fifth year of the program, awards are being made to twenty-one institutions for programs that collectively span the areas of science and engineering supported by
NSF.
ABSTRACT Couzis, Alexander et al
CUNY City College
“Sensors: Biosensor Arrays from Intact Receptor Proteolipsomes Immobilized onto Surfaces”
The dominant receptor family involved in molecular sensing and signal transduction by the cell are the G protein-coupled membrane receptors (GPCRs) which are responsible for detecting extracellular molecular signals and transducing the signals via the coupled heterotrimeric G protein. GPCRs sense an expansive range of ligands, including neurotransmitters, odorant molecules and growth factors. The
GPCR sensor molecular machinery is therefore a natural system to be used in the design of biosensors. The aim of this research grant is to develop biomimetic sensor arrays composed of GPCRs. The primary limitation with using GPCRs as a sensing element is that these receptors require a specialized lipid bilayer membrane environment to maintain activity ex vivo. Previous efforts in this direction have incorporated the GPCRs into planar bilayer structures on surfaces in order to fulfill the requirement of a lipid environment to maintain
GPCR activity. The research approach of this grant is based on the immobilization of intact
GPCR-liposomes (proteoliposomes) onto nanostructured surfaces with domains of controlled terminal functionality. These liposomes will contain the membrane protein receptors in the lipid bilayer and, more importantly, also encapsulate G proteins and their downstream targets such as phospholipase C, in the core of the liposome. This approach stands to significantly enhance the senstivity of
GPCR arrays by harnesing the natural applification mechanisms of the cell due to the potential for encapsulation inside the proteoliposome. In principle, the design can be scaled-up using microfluidic concepts to explore the interactions in a multitude of combinations of ligand, receptor, G proteins and their downstream effectors.
The team consists of investigators with an established record of collaboration from the City College of New York, combining backgrounds in biophysics, bioengineering, interfacial engineering, biochemistry, chemical engineering, and genetics. It is the intention of this team to develop the science and required technology to achieve such a design and along the way provide a unique, multidisciplinary educational experience for the graduate, undergraduate, and post-graduate students that will be involved.
Furthermore, this provides a unique opportunity to interface graduate and undergraduate training efforts in surface science (
NSF 9972892) and soft materials (
NSF 0221589) already funded by the
NSF with biochemistry training efforts funded by the
NIH (
RCMI,
MARC, &
RISE) that would otherwise function independently of one another. All the co-PIs are involved in one or more of the above mentioned efforts. These training efforts will provide additional resources such as undergraduate research funding and first-year graduate student funding. In addition, this proposal will take advantage of the recent successful efforts to secure infrastructure funding from the
NSF and the DoD.
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